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Restorase® DNA Polymerase with 10× Reaction Buffer

产品编号:3215032
规格:Enzyme blend for PCR amplification of damaged DNA
包装规格:50 REACTIONS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
321503250 REACTIONS3270
基本信息
NACRES
NA.55
General description【一般描述】
Restorase DNA Polymerase with 10× Reaction Buffer combines Sigma′s long and accurate enzyme technology with a small amount of DNA repair enzyme. The optimized blend will initiate the repair and further amplification of damaged DNA templates greater than 800 bp. Restorase has also been shown to increase yield on undamaged DNA templates.
Application【应用】
Restorase® DNA Polymerase with 10× Reaction Buffer has been used in DNA repair.
Biochem/physiol Actions【生化/生理作用】
DNA templates are often damaged on exposure to heat, acids, alkylating agents, and light. These damaged templates result in inefficient or failed DNA amplification by polymerase chain reaction (PCR). Restorase® DNA Polymerase repairs the damaged sites on the DNA templates and initiates subsequent template copying. Restorase treatment of the DNA depends on the level of template damage.
Features and Benefits【特点和优势】
  • Reliable amplification of damaged DNA
  • When thermostable polymerases fail, this enzyme amplifies the sequence efficiently
  • Efficient amplification of sequences in multiplex reactions
  • Increased yield and amplicon specificity
  • Amplifies a broad spectrum of amplicon sizes varying from 200 bp to 20 kb
  • Can repair 3′ bungs, nicks, and abasic sites
Other Notes【其他说明】
View more detailed information on Restorase DNA Polymerase at www.sigma-aldrich.com/restorase.
Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.
Legal Information【法律信息】
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Restorase is a registered trademark of sigma-aldrich Co. LLC
产品性质
Quality Level【质量水平】
200
form【形式】
liquid
usage【用途】
sufficient for 50 reactions
feature【特点】
Long & Accurate PCR
dNTPs included: no
hotstart: no
concentration【浓度】
2.5 units/μL
technique(s)
PCR: suitable
color【颜色】
colorless
input
purified DNA
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
packaging【包装】
The enzyme is provided with an optimized 10× reaction buffer provided as 1 vial/250 units.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 3
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