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基本信息
| eCl@ss | 32190102 |
| Cell Line Origin【细胞系来源】 | Rabbit kidney |
| Cell Line Description【细胞系描述】 | RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line. |
| Application【应用】 | Virus studies |
| Culture Medium【培养基】 | EMEM(HBSS) (M5775) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ºC; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
产品性质
| biological source【生物来源】 | rabbit kidney |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n=44, stem-line = 56 (=22%) |
| morphology【形态学】 | Epithelial-like |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
