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凝血酶 来源于牛血浆
Thrombin from bovine plasma
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| 产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
| 3251916 | 100 UNITS | 1220 | |||
| 3251916 | 250 UNITS | 2400 | |||
| 3251916 | 500 UNITS | 3400 | |||
| 3251916 | 1000 UNITS | 6460 |
产品别名
9002-04-4
凝血酶 来源于牛血浆
Thrombin from bovine plasma
Factor IIa
IIa 因子
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.4.21.5 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 232-648-7 |
| MDL number | MFCD00166053 |
| NACRES | NA.54 |
| General description【一般描述】 | Thrombin is a sodium-activated type II enzyme. It contains two anion binding exosites, ABE-I and ABE-II. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond. The B chain of a-thrombin includes a carbohydrate portion. |
| Application【应用】 | Thrombin from bovine plasma has been used to study its effect on the perinatal rat subventricular zone cells and oligodendrocyte precursor cell proliferation, differentiation, and migration in culture. It has also been used in fibrin degradation assay to measure nattokinase activity. 凝血酶用于重组融合蛋白的位点特异性切割，所述重组融合蛋白包含用于去除亲和标签的可接近凝血酶识别位点。凝血酶已被用于一项评估全球止血和护理点测试的研究。 |
| Biochem/physiol Actions【生化/生理作用】 | Thrombin is a proteolytic enzyme critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. Thrombin promotes platelet aggregation. Therefore, thrombin is the final coagulation protease in hemostasis, promoting both procoagulant and anticoagulant effects. It is used to treat bleeding from capillaries and small venules. 丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。 |
| Unit Definition【单位定义】 | 活性以NIH单位表示，通过与NIH凝血酶参考标准品直接对比而获得。 |
| Physical form【外形】 | 由生理盐水柠檬酸钠缓冲液，pH 6.5，制得冻干粉 |
| Analysis Note【分析说明】 | 活性是通过与一种K批次的NIH凝血酶参考标准品进行直接比较而得到并以NIH单位进行表达的。 基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆(由血浆与等量生理盐水混合制得)作为底物，以及 0.1mL 凝血酶样品(在含 1% 白蛋白缓冲液中保持稳定)。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。 |
| Other Notes【其他说明】 | 查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。 |
产品性质
| Quality Level【质量水平】 | 300 |
| form【形式】 | lyophilized powder |
| Specific Activity | 600-2,000 NIH units/mg protein (biuret) |
| mol wt【分子量】 | heavy chain ~33 kDa light chain ~5 kDa |
| UniProt accession no.【UniProt登记号】 | P00735 |
| application(s) | diagnostic assay manufacturing |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | cow ... F2(280685) |
安全信息
| Pictograms【象形图】 | GHS07,GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H315 - H319 - H334 - H335 |
| Precautionary Statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Classifications【危险分类】 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
| Target Organs【靶器官】 | Respiratory system |
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 2 |
