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产品性质
| biological source【生物来源】 | Mouse x Mouse Hybridoma |
| ImmunoDon | BALB/c mouse spleen |
| Myeloma【骨髓瘤】 | P3-NS1/1-Ag4.1 |
| Reactivity【反应性】 | Human ′A′ erythrocytes |
| technique(s) | cell culture | mammalian: suitable |
| isotype【同位素/亚型】 | IgM |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Description【细胞系描述】 | The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A. |
| Immunogen【免疫原】 | Human tonsil membrane |
| Application【应用】 | Human erythrocyte marker. |
| Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) |
| Subculture Routine【传代培养常规】 | Seed at 5 x 104 cells/ml. Subculture when cells reach 5 x 105 -1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37℃. Start in 20% FBS when recovering from frozen. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
