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产品性质
| biological source【生物来源】 | human spleen |
| growth mode【生长模式】 | Suspension |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Lymphoblast |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human Caucasian B lymphocyte |
| Cell Line Description【细胞系描述】 | A non Ig-secreting, HAT sensitive, 6-thioguanine resistant, human B lymphocyte. Derived from the parent line WIL2.NS (Sigma Catalogue number. 90112121). |
| Application【应用】 | Immortalising antigen-stimulated human B-lymphocytes by somatic hybridisation |
| Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Maintain cultures between 5x1,000-5x10,000 cells/ml; 5% CO2; 37℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
