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产品性质
| biological source【生物来源】 | mouse embryo |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n = 40; hypertriploid |
| morphology【形态学】 | Fibroblast |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| General description【一般描述】 | Product has lost contact inhibition |
| Cell Line Origin【细胞系来源】 | Mouse Swiss Albino embryo fibroblast |
| Cell Line Description【细胞系描述】 | Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition. |
| Application【应用】 | Contact inhibition and virus studies: polyoma and SV40 viruses |
| Culture Medium【培养基】 | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
