5-DBCO-PEG₄-dUTP, 10mM Aqueous Solution

C₄₂H₅₄N₅O₂₁P₃

1057.82

329769180

NA.22

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.

200

>95% (HPLC)

solution

reaction type: click chemistry

Tris buffer: 100 mM (pH 7.5)

wet ice

−20℃

O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O

50 μL in poly bottle

12 - Non Combustible Liquids

WGK 2