α-Actinin from chicken gizzard

MFCD00130473

NA.32

α-Actinin exists as a rod-shaped antiparallel dimer with two elongated subunits. The domain region contains an N-terminal actin-binding domain with tandem calponin homology domains, a central tandem 3-helix motifs, and EF-hand motifs at C-terminus. It belongs to the spectrin superfamily and is localized in actin structures.

α-Actinin from chicken gizzard has been used:

• as an antigen to coat plates for the capture of anti-actinin antibody using enzyme-linked immunosorbent assay (ELISA) from tumor cell line clones
• in in vitro motility experiments to test its effect on actin filament movement
• to coat cantilevers for strengthening fluorescently labeled actin filaments in force measurement studies

α-Actinin binds actin and has the ability to crosslink actin cytoskeleton. It also plays a key role in the structural maintenance of the Z-disk of striated muscle.

Suspension in 2 M (NH₄)₂SO₄ containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride

Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations.

Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979).

chicken gizzard

200

~80% α-actinin basis (SDS-PAGE)

ammonium sulfate suspension

100 kDa

P05094
P12814
P20111
Q7TPR4
Q90734
Q9Z1P2

2-8℃

chicken ... ACTN2(396263), ACTN4(396024), RCJMB04_23c5(373918)
human ... ACTN1(87)
mouse ... ACTN1(109711)
rat ... ACTN1(81634)

5 mg in poly bottle
Package size based on protein content

10 - Combustible liquids

WGK 3

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)