Achromopeptidase 来源于细菌

3.4.21.50 ( BRENDA | IUBMB )

MFCD00130333

NA.54

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.
Achromopeptidase from bacteria may be used:

• in the homogenization step for genomic DNA extraction from microbial biofilm
• in the lysis of gram-positive strains(
• for DNA extraction from Nocardia strains

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀ = 0.6 over 2 hours at 37 ℃.
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

One unit will produce a change in A₆₀₀ of 0.001 per minute per mL at pH 8.0 at 37 ℃ using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).

Partially purified powder.

200

partially purified powder

≥20,000 units/mg solid

27 kDa

Collagenase, present

−20℃

GHS08

Danger

H334

P261 - P342 + P311

Resp. Sens. 1

13 - Non Combustible Solids

WGK 3

dust mask type N95 (US), Eyeshields, Faceshields, Gloves