Alkaline Phosphatase Blue Membrane Substrate Solution

42010105

NA.83

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:

• to analyse direct alkaline phosphatase (AP) activity
• to perform alkaline phosphatase assays with colorimetric system
• to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3

Prior to reaction with alkaline phosphatase, the BCIP^® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP^®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

BCIP is a registered trademark of sigma-aldrich Co. LLC

200

solution

sufficient for 400 mL working substrate

2-8℃

12 - Non Combustible Liquids