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Aminopeptidase I from Streptomyces griseus
产品别名
9031-94-1
Aminopeptidase I from Streptomyces griseus
Leucine Aminopeptidase IV
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.4.11.22 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 232-874-6 |
| MDL number | MFCD00166325 |
| NACRES | NA.54 |
| General description【一般描述】 | Aminopeptidase I from Streptomyces griseus is a thermostable enzyme with Glu131 and Tyr246 as key active site residues. |
| Application【应用】 | Aminopeptidase I from Streptomyces griseus has been used:
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| Biochem/physiol Actions【生化/生理作用】 | Aminopeptidase I may also be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. In the first stage, the endoprotease cleaves a peptide, such as Z-Y-X-Leu-p-nitroanilide, with the X, Y, and Z residues being chosen according to the specificity of the endoprotease. Aminopeptidase I from S. griseus has a fairly broad specificity, being able to remove the N-terminal residue of most proteins, except where the penultimate residue is an imino acid. It contains two Zn2+ binding sites. Aminopeptidase I from S. griseus is inhibited by 1,10-phenanthroline and is activated six-fold by Ca2+, which also stabilizes it against heat inactivation. This monomeric zinc metalloprotein has an isoelectric point (pI) of 5.4. |
| Unit Definition【单位定义】 | One unit will hydrolyze 1.0 μmole of |
| Physical form【外形】 | Contains calcium acetate |
| Preparation Note【制备说明】 | Reconstitute in 20 mM tricine, pH 8.0, with 0.05% bovine serum albumin. Dilute the enzyme with the reconstitution buffer to 0.15-0.3 U/mL for a working concentration. Solutions should be prepared fresh prior to use. |
| Other Notes【其他说明】 | Endopeptidase contaminant: Not more than: 0.01 U/mg protein (as μmole tyrosine equivalent per min released from casein.) |
产品性质
| form【形式】 | lyophilized powder |
| Quality Level【质量水平】 | 200 |
| Specific Activity | ≥200 units/mg protein |
| mol wt【分子量】 | 21 kDa by gel filtration 33 kDa by SDS-PAGE |
| composition【组成】 | Protein, 40-60% Lowry |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 0.1 mg in serum bottle Package size based on protein content. |
安全信息
| Pictograms【象形图】 | GHS07,GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H315 - H319 - H334 - H335 |
| Precautionary Statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Classifications【危险分类】 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
| Target Organs【靶器官】 | Respiratory system |
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
