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Anti-AFX (FOXO4) antibody, Mouse monoclonal
| 产品编号： | 3343727 |
| 规格： | clone AF3.10, purified from hybridoma cell culture |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD03791410 |
| NACRES | NA.41 |
| General description【一般描述】 | Forkhead box O4 (FOXO4) belongs to the FOXO transcription factor family that regulates growth, cell cycle, metabolism, stress tolerance, aging and apoptosis in cells. Studies have reported that USP7 modulates FOXO4 transcription. Moreover, it has been demonstrated that peroxide stress acetylates FOXO4, whereas the longevity protein hSir2SIRT1 deacetylates it. |
| Specificity【特异性】 | Monoclonal Anti-AFX (FOXO4) recognizes human AFX. |
| Application【应用】 | Monoclonal Anti-AFX (FOXO4) antibody can be used for western blot assays at 0.5-1μg/ml. The antibody is also suitable for use in indirect ELISA, immunocytochemistry and microarray applications. |
| Biochem/physiol Actions【生化/生理作用】 | Anti-AFX (FOXO4) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma AF3.10 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. AFX plays a central role in the regulation of cell proliferation. It integrates signals from PI3K/PKB and Ras/Ral signaling pathways to regulate transcription of the cell cycle inhibitor p27kip1. AFX activation results in increased expression of p27kip1, resulting in cell cycle arrest. Overexpression of AFX blocks cells cycle progression at G1 by a p27kip1 dependent mechanism. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | AF3.10, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~50 kDa |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable indirect ELISA: suitable microarray: suitable western blot: 0.5-1 μg/mL using COS-7 cell extracts expressing human AFX |
| isotype【同位素/亚型】 | IgG2b |
| UniProt accession no.【UniProt登记号】 | P98177 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... FOXO4(4303) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
