Anti-Androgen Receptor antibody produced in rabbit

MFCD01321686

NA.41

Androgens are the male sex hormones that exert biological and transcriptional effects on binding to nuclear androgen receptor (AR). Androgens in humans include androstenedione, testosterone, dihydrotestosterone and dehydroepiandrosterone. Androgen receptor is a ligand inducible nuclear receptor, expressed on brain, bone, skeletal muscles, genital organs, hair follicles and cardiovascular tissue. On binding androgen, the AR is internalized and modulates the transcriptional of target genes. Additionally, AR also exerts extra-nuclear actions wherein it rapidly induces signalling cascades of MAPK and protein kinase C. The regulation by AR results in irreversible sexual differentiation of sex organs during embryogenesis and development of sex-specific characteristics during puberty
Anti-Androgen Receptor specifically recognizes the human androgen receptor protein (110 kDa). An additional band may be observed at 45 kDa. The antibody cross-reacts with rat androgen receptor.

The antibody cross-reacts with androgen receptor from rat.

evolutionally conserved peptide corresponding to amino acids 1-21 of the human androgen receptor. This sequence is not found in estrogen, progesterone, or glucocorticoid receptors.

Rabbit anti-androgen receptor (C19) was used in a EMSA (electrophoretic mobility shift assay) which produced a supershift in the molecular weight of a particular DNA sequence indicating binding of the androgen receptor.
Anti-androgen receptor antibody may be used for immunoblotting using whole rat brain extract at a recommended concentration of 1-2 μg/ml. For immunoprecipitaion from 250 μg of human MCF7 cell lysate, a concentration of 5-10 μg may be used. Immunohistochemistry may be performed on paraffin-embedded human prostate tissue sections at a working antibody concentration of 20-40 μg/ml.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen 110 kDa (additional band may be observed at 45 kDa)

rat, human

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using microwave-pretreated human prostate tissue sections
immunoprecipitation (IP): 5-10 μg using human MCF7 cell lysate ( 250 μg)
microarray: suitable
western blot: 1-2 μg/mL using whole extract of rat brain

P10275

dry ice

−20℃

human ... AR(367)
rat ... Ar(24208)

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WGK 2

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