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Anti-Cortactin (GK-18) antibody produced in rabbit

产品编号:3354810
规格:IgG fraction of antiserum, buffered aqueous solution
包装规格:200 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3354810200 μL7370
产品别名

Anti-Cortactin (GK-18) antibody produced in rabbit

Anti-EMS1

Anti-p80/85

基本信息
MDL number
MFCD03791486
NACRES
NA.41
General description【一般描述】
Cortactin gene is located on human chromosome 11q13. Cortactin exists as multiple isoforms (75-85 kDa), formed by alternative splicing. It contains an unique multidomain structure consisting of 6.5 tandem repeats of a 37-amino acid sequence required for direct binding to F-actin, followed by an α-helix region, a proline-rich region, and three tyrosine residues targeted by sarcoma (Src) related kinases. It represents a highly conserved family of perimembrane signaling proteins, including the hematopoietic lineage cell specific proteins hematopoietic cell-specific Lyn substrate 1 (HS1/LCKBP1) and hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa (HIP-55).
Immunogen【免疫原】
synthetic peptide corresponding to the N-terminal region of human cortactin (amino acids 18-35) conjugated to KLH.
Application【应用】
Anti-Cortactin (GK-18) antibody produced in rabbit is suitable for the following applications:
  • Indirect immunofluorescence at a dilution of 1:500 using mouse fibroblasts NIH3T3 cell line
  • Microarray
  • Western blotting at a dilution of 1:1,000 using cytosol extract of rat brain, whole extract of chicken embryo fibroblasts or whole cell extract of human epidermoid carcinoma A431 cell line
Biochem/physiol Actions【生化/生理作用】
Cortactin (EMS1) is an important branched actin regulator involved in invasion and migration of tumor cells and hepatocellular carcinoma (HCC). Overexpression of this protein is closely associated with poor human HCCs prognosis caused by cancer embolus and metastasis. The overexpressed protein promotes vesicular trafficking at a variety of cellular sites including endosomes and the trans-Golgi network. Trafficking is highly sensitive to cortactin-regulated branched actin assembly.
Cortactin is overexpressed in human breast cancer and in head and neck tumors. Overexpression and redistribution of cortactin into podosome-like structures has been observed in human carcinoma cell lines suggesting that cortactin is involved in tumor cell invasion and metastasis. The C-terminal region contains a Src-homology 3 (SH3) domain that interacts with several postsynaptic density (PSD), PDZ-family (post-synaptic density protein 95 (PSD-95), Drosophila disc large tumor suppressor (Dlg1) and zona occludens 1 (ZO-1)) proteins) including cortactin-binding protein 1 (CortBP1), SH3 and multiple ankyrin repeat domains 3 (SHANK3), Zonula occludens-1 (ZO-1) and Cortactin-binding protein 90 (CBP90) (CBP-90). Cortactin is enriched within lamellipodia of motile cells and in neuronal growth cones. Cortactin is normally phosphorylated on serine and threonine residues. Cortactin becomes heavily phosphorylated on tyrosine in pp60src transfected oncogenic fibroblasts and accumulates with F-actin in podosomes. Cortactin co-localizes with the actin-related protein Arp2/3 complex at sites of actin polymerization within the lamellipodia It binds directly to the Arp2/3 complex and activates it to promote assembly of branched actin filaments networks.
Physical form【外形】
Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
200
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
IgG fraction of antiserum
antibody product type
primary antibodies
clone【克隆】
polyclonal
form【形式】
buffered aqueous solution
species reactivity
mouse, rat, chicken, human
technique(s)
indirect immunofluorescence: 1:500 using mouse fibroblasts NIH3T3 cell line
microarray: suitable
western blot: 1:1,000 using cytosol extract of rat brain, whole extract of chicken embryo fibroblasts, or whole cell extract of human epidermoid carcinoma A431 cell line
UniProt accession no.【UniProt登记号】
Q14247
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... CTTN(2017)
mouse ... Cttn(13043)
rat ... Cttn(60465)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
nwg
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