Anti-Corticotropin Releasing Factor antibody produced in rabbit

MFCD02096145

NA.41

Corticotropin Releasing Factor (CRF), a 41 amino acid neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is derived from a larger 196 amino acids precursor protein (preproCRF). CRF is widely distributed in the brain and peripheral nervous system, with the highest levels in the hypothalamic paraventricular nucleus (PVN).

The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, rat urocortin, PACAP38, human ACTH, human ACTH (18-39) and sauvagine.

synthetic peptide corresponding to the C-terminal of human CRF (amino acids 25-41 with N-terminally added lysine) conjugated to KLH.

Anti-Corticotropin Releasing Factor antibody produced in rabbit has been used in immunohistochemistry (1:2000) and radioimmunoassay.

Corticotropin Releasing Factor (CRF) is critical in the regulation of the hypothalamic-pituitary-adrenal gland axis, and autonomic and behavioral responses to stress. CRF effects include changes in motor and cognitive behavior, appetite control and modulation of the immune system. CRF is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion by corticotroph cells from the anterior pituitary in vitro and in vivo. The effects of CRF are mediated through CRF receptor subtypes CRFR1 and CRFR2 (splice variants). CRF binds with a high affinity to CRF-binding protein (CRF-BP), which inactivates CRF, while ligands that dissociate CRF from CRF-BP exhibit cognition-enhancing properties. CRF overproduction has been implicated in anorexia and depression and may lead to Cushing′s syndrome. CRF levels are correlated with Alzheimer′s Disease (AD) pathogenesis.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

rabbit

200

unconjugated

IgG fraction of antiserum

primary antibodies

polyclonal

buffered aqueous solution

bovine, mouse, frog, sheep, canine, rat, pig, human

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using microwave-treated sections of human placental tissue
microarray: suitable
radioimmunoassay: 1:10,000 using 2-¹²⁵I-His¹²-CRF, human

P06850

dry ice

−20℃

human ... CRH(1392)
mouse ... Crh(12918)
rat ... Crh(81648)

For continuous use, store at 2-8℃ for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

nwg