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Anti-eIF2α 兔抗
Anti-eIF2α antibody produced in rabbit
![](/image/daily/2018/01/10/122206_412042_proimg_3359461.jpg)
产品编号: | 3359461 |
规格: | IgG fraction of antiserum, buffered aqueous solution |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-eIF2α 兔抗
Anti-eIF2α antibody produced in rabbit
Anti-EIF2
Anti-Eukaryotic translation initiation factor 2, subunit 1
Anti-EIF2S1
Anti-EIF-2A
Anti-Eukaryotic translation initiation factor 2
Anti-EIF-2
Anti-eIF-2-alpha
基本信息
NACRES | NA.41 |
General description【一般描述】 | The eukaryotic translation initiation factor 2 subunit |
Specificity【特异性】 | Anti-eIF2α (also known as eIF-2A) recognizes human eIF2α. |
Application【应用】 | Anti-eIF2α antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:250-1:500 using PC-12 cell lysate. It was used as a primary antibody to detect eIF2α in a study. Anti-eIF2α antibody produced in rabbit has been used in western blotting. |
Biochem/physiol Actions【生化/生理作用】 | The main role of eIF2 complex is to transfer Met-tRNAi in the presence of GTP to the 40S ribosomal subunit during the initiation of translation. Regulation of the initiation of translation at the level of eIF2 involves the phosphorylation of eIF2α at a conserved Ser51 residue. This leads to inhibition of eIF2β activity, thus reducing guanine nucleotide exchange and consequently, the translation rate. Protein synthesis inhibition at the level of eIF2α phosphorylation is linked to a variety of cell stress signaling pathways via specific kinases. Phosphorylation of eIF2α reduces the rate of translation and gives the cell time to correct the stress damage, and selectively enhance the translation of genes important for stress remedy. Increased eIF2α phosphorylation has been observed in Alzheimer′s disease. Viral infections are also known to induce eIF2α phosphorylation. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | IgG fraction of antiserum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~35 kDa |
species reactivity | human |
technique(s) | western blot: 1:250-1:500 using PC-12 cell lysate |
UniProt accession no.【UniProt登记号】 | P05198 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... EIF2S1(1965) |
产品说明
Storage and Stability【储存及稳定性】 | For continuous use, store at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |