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Anti-eIF2Bβ (N-terminal) antibody produced in rabbit

产品编号:3359500
规格:~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
包装规格:200 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3359500200 μL4730
产品别名

Anti-eIF2Bβ (N-terminal) antibody produced in rabbit

Anti-EIF2B-beta

Anti-Eukaryotic translation initiation factor 2B, beta

Anti-EIF2BB

Anti-EIF-2B

Anti-Eukaryotic translation initiation factor 2B, subunit 2

基本信息
NACRES
NA.41
General description【一般描述】
Translation initiation factor eIF-2B subunit beta eIF2B β is a component of the regulatory subcomplex of eIF2B along with the eIF2Bα and -δ subunits. eIF2Bβ gene is mapped to human chromosome 14q24.3.
Specificity【特异性】
Anti-eIF2BNβ-terminal) specifically recognizes human, mouse, and rat eIF2Bβ.
Application【应用】
Anti-eIF2Bβ (N-terminal) antibody produced in rabbit is suitable for the following applications:
  • Immunoprecipitation at a concentration of 3-5μg using K562 cell lysates
  • Indirect immunofluorescence (at a concentration of 2-5μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human eIF2Bβ)
  • Western blotting at a concentration of 2-4μg/mL using K562 or AT3B1 cell lysates
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if
Biochem/physiol Actions【生化/生理作用】
Recent studies have linked inherited mutations in any of the five eIF2B subunits to a fatal human disorder known as childhood ataxia with central nervous system hypomyelination (CACH) or vanishing white matter (VWN) disease. The eIF2B -α, -β and - δ subunits downregulate eIF2B activity in response to the phosphorylation of eIF2 on Ser51.
Translation initiation factor eIF-2B subunit β is a protein encoded by EIF2B2 gene in humans. It is a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. It is associated with the cause of autosomal recessive disorders. Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities called eIF2B-related disorders.
Physical form【外形】
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
200
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
affinity isolated antibody
antibody product type
primary antibodies
clone【克隆】
polyclonal
form【形式】
buffered aqueous solution
mol wt【分子量】
antigen ~39 kDa
species reactivity
human, mouse, rat
concentration【浓度】
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 3-5 μg using K562 cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human eIF2Bβ
indirect immunofluorescence: suitable
western blot: 2-4 μg/mL using K562 or AT3B1 cell lysates
UniProt accession no.【UniProt登记号】
P49770
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... EIF2B2(8892)
mouse ... Eif2b2(217715)
rat ... Eif2b2(84005)
产品说明
Storage and Stability【储存及稳定性】
For continuous use, store at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer【免责声明】
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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