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Anti-eIF2Bε antibody produced in rabbit
| 产品编号： | 3359501 |
| 规格： | IgG fraction of antiserum, buffered aqueous solution |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-eIF2Bε antibody produced in rabbit
Anti-eIF-2B GDP-GTP exchange factor subunit epsilon
Anti-Eukaryotic translation initiation factor 2B, subunit 5
Anti-Eukaryotic translation initiation factor 2B, ε
基本信息
| NACRES | NA.41 |
| Immunogen【免疫原】 | synthetic peptide corresponding to amino acids 50-65 of human eIF2ε conjugated to KLH. The corresponding sequence is identical in rat and mouse. |
| Application【应用】 | Anti-eIF2Bε antibody produced in rabbit is suitable for indirect immunofluorescence (at a dilution of 1:500-1:1,000 using paraformaldehyde fixed NIH-3T3 cells) and western blotting at a dilution of 1:500-1:1,000 using Rat1 or HEK-293T cell lysates. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if. |
| Biochem/physiol Actions【生化/生理作用】 | Translation initiation factor eIF-2B subunit ε is a protein encoded by the EIF2B5 gene in humans. The eIF2B is composed of five subunits α, β, γ, δ and ε, within which the ε subunit is responsible for catalyzing the guanine exchange reaction. Defect in any of these 5 subunits leads to diseases. Mutation in the subunit of eIF2B is associated with an autosomal recessive leukoencephalopathy called Vanishing white matter (VWM). |
| Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~80 kDa |
| species reactivity | mouse, rat, human |
| technique(s) | indirect immunofluorescence: 1:500-1:1,000 using paraformaldehyde fixed NIH-3T3 cells. indirect immunofluorescence: suitable western blot: 1:500-1:1,000 using Rat1 or HEK-293T cell lysates. |
| UniProt accession no.【UniProt登记号】 | Q13144 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... EIF2B5(8893) mouse ... Eif2b5(224045) rat ... Eif2b5(192234) |
产品说明
| Target description【目标描述】 | eIF2Bε encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
