Anti-GABA_B Receptor 2 兔抗

MFCD03455041

NA.41

The GABAB receptor (GABABR) is a heteromeric G-protein-coupled receptor. It has two subunits-GABAB1 (γ-aminobutyric acid type B receptor subunit 1) and GABAB2 (γ-aminobutyric acid type B receptor subunit 2). In the hippocampus, GABAB2 is present in dendrites and located in the extra-synaptic membrane of spines and dendritic shafts. This gene is located on rat chromosome 5q.

peptide CKDPIEDINSPEHIQRRLSL corresponding to residues 875-894 of human GABA_BR2.

Anti-GABAB Receptor 2 antibody has been used in western blotting.
Anti-GABA_B Receptor 2 antibody produced in rabbit is suitable for western blotting at a working dilution of 1:200 using rat brain membranes.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

The inhibitory neurotransmitter GABA signals through two distinct types of pre- and postsynaptic receptors, GABA_A and GABA_B. Both GABA receptors can regulate depression of synaptic transmission and be involved in the inhibition controlling neuronal excitability. The GABA transmitter interacts with three types of receptors: the ionotropic receptors GABA_A and GABA_C and the metabotropic GABA_B receptor (GABABR). GABABR is a G-protein coupled receptor that plays a key role in slow synaptic inhibition in the brain and spinal cord. GABABR is a heteromer with two subunits, GABABR1 and GABABR2. The amino acids within the second intracellular domain of GABA(B2) are essential for the coupling of GABA(B) receptor heterodimers to their downstream effector systems. The N-termini on both the GB1 and GB2 receptors are involved in the normal coupling of GABAB receptors to their physiological effectors, G(i) and G-protein-activated potassium channels (GIRKs).

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%).

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

lyophilized powder

rat

western blot: 1:200 using rat brain membranes

O88871

dry ice

−20℃

rat ... Gabbr2(83633)

The GABA_BR2 was shown to be essential for trafficking and G-protein binding, while the GABA_B R1 subunit was demonstrated to be important for agonist and antagonist binding. GABA_BR2 is highly expressed in brain especially at the cerebral cortex, thalamus, hippocampus, and spinal cord and to a lesser extent in testis.

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13 - Non Combustible Solids

WGK 2