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抗 谷胱甘肽-S-转移酶
Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit
| 产品编号： | 3365111 |
| 规格： | IgG fraction of antiserum, buffered aqueous solution |
| 包装规格： | 0.5 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
抗 谷胱甘肽-S-转移酶
Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit
Anti-Glutathione-S-Transferase
Anti-GST
抗 GST
基本信息
| NACRES | NA.46 |
| General description【一般描述】 | 该抗体对日本血吸虫 谷胱甘肽-S-转移酶的天然型和变性-还原型均具有特异性 。抗 GST 可用于各种免疫分析，以鉴定 GST 融合蛋白的表达。 Glutathione-S-Transferase (GST) belongs to a family of Phase II detoxification enzymes which are involved in catalyzing the conjugation of glutathione (GSH) with many exogenous and endogenous electrophilic substances. GST is of two kinds- membrane-bound microsomal and cytosolic. The cytosolic GSTs are made of six classes: α, μ, ω, π, θ, and ζ. |
| Specificity【特异性】 | Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins. |
| Immunogen【免疫原】 | 日本血吸虫 的重组 GST 在 大肠杆菌中表达 。 |
| Application【应用】 | Anti-Glutathione-S-Transferase (GST)-Peroxidase Conjugate antibody has been used in
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions【生化/生理作用】 | Glutathione-S-Transferase (GST) are involved in protecting cellular macromolecules from the reactive electrophiles. The μ and π transferases regulate the mitogen-activated protein (MAP) kinase pathway. GSTs help in building up cellular resistance against microbial antibiotic, herbicides, insecticides, chemotherapy agents and drugs. |
| Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT. |
| Preparation Note【制备说明】 | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | peroxidase conjugate |
| antibody form【抗体形式】 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen 27.5 kDa |
| technique(s) | direct ELISA: 1:10,000 western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Pictograms【象形图】 | GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H317 - H334 |
| Precautionary Statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Classifications【危险分类】 | Resp. Sens. 1 - Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
