Anti-Golph3 (N-terminal) antibody produced in rabbit

NA.41

Golph3 (Golgi phosphoprotein 3), also known as coat protein GPP34, is a peripheral membrane protein of the Golgi stack, that localizes to the trans-Golgi network. It is an oncogene that is commonly targeted for amplification in human cancer and in cancer cell lines.

peptide corresponding to the N-terminal region of human Golph3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and bovine.

Anti-Golph3 (N-terminal) antibody produced in rabbit has been used in:

• immunoprecipitation
• immunofluorescence
• immunoblotting

Golph3 (Golgi phosphoprotein 3) is important for Golgi trafficking and morphology by interacting with the unconventional myosin MYO18A, linking Golgi membranes to the actin cytoskeleton. It is a phosphatidylinositol-4-phosphate (PtdIns(4)P binding protein required for Golgi function. GOLPH3 overexpression is correlated with hyperactivation of mTOR signaling, in human cells.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~34 kDa

mouse, human

~1.0 mg/mL

immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells
indirect immunofluorescence: 2.5-5.0 μg/mL using mouse 3T3 cells
western blot: 1-2 μg/mL using whole extracts of human HeLa cells

Q9H4A6

dry ice

−20℃

human ... GOLPH3(64083)
mouse ... Golph3(66629)
rat ... Golph3(78961)

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12 - Non Combustible Liquids