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Anti-HRD1/SYVN1 antibody produced in rabbit
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产品编号: | 3368446 |
规格: | ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-HRD1/SYVN1 antibody produced in rabbit
Anti-Synoviolin
Anti-Synovial apoptosis inhibitor 1
Anti-Synoviolin 1
基本信息
NACRES | NA.41 |
General description【一般描述】 | Synoviolin (SYVN1) is an endoplasmic reticulum (ER)-membrane resident E3 ubiquitin ligase. The SYVN1 gene is mapped to human chromosome 11q13.1. Also known as HRD1, this protein has five transmembrane domains and a really interesting new gene (RING) finger domain. |
Specificity【特异性】 | Anti-HRD1/SYVN1 recognizes human and mouse HRD1/SYVN1. |
Application【应用】 | Anti-HRD1/SYVN1 antibody produced in rabbit has been used in immunoblotting and immunoprecipitation. |
Biochem/physiol Actions【生化/生理作用】 | Synoviolin (SYVN1) protects against endoplasmic reticulum (ER) stress-induced apoptosis through ER stress-associated protein degradation (ERAD). The really interesting new gene (RING) finger domain of SYVN1 mediates the transfer of ubiquitin from E2 to substrates and a proline-rich domain. E3 ubiquitin-protein ligase (HRD1) expression is strongly induced by ER stress. HRD1 interacts with parkin-associated endothelin receptor-like receptor (Pael-R), promoting its degradation, and protects neurons from cell death caused by the accumulation of Pael-R. HRD1 is also found to enhance the degradation and suppress the toxicity of polyglutamine-expanded huntingtin. In addition to its role in ERAD, HRD1 targets tumor suppressor gene p53 for proteasomal degradation. Overexpression of HRD1 is implicated in the pathogenesis of rheumatoid arthritis. The deletion of gene locus of SYVN1 is implicated in spastic paraplegia, optic atrophy, and neuropathy (SPOAN) disorder. |
Physical form【外形】 | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~70 kDa |
species reactivity | mouse, human |
concentration【浓度】 | ~1.0 mg/mL |
technique(s) | immunoprecipitation (IP): 5-10 μg using lysate of mouse brain indirect immunofluorescence: suitable western blot: 2-4 μg/mL using whole extract of HEK-293T cells expressing human HRD1/SYVN1. |
UniProt accession no.【UniProt登记号】 | Q86TM6 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... SYVN1(84447) mouse ... Syvn1(74126) rat ... Syvn1(361712) |
产品说明
Storage and Stability【储存及稳定性】 | For continuous use, store at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots at –20 ℃. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |