Anti-INCENP antibody produced in rabbit

MFCD05665235

NA.41

The gene INCENP (inner centromere protein) encodes an inner centromere protein that forms a component of the chromosomal passenger complex (CPC). It is found to be localized to inner centromeres during mitosis. The CPC also contains Aurora B, survivin, and Borealin.

synthetic peptide corresponding to amino acid residues 884-901 of human INCENP with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in chicken and Xenopus laevis and by two amino acids in mouse.

Anti-INCENP antibody produced in rabbit has been used in western blotting and immunofluorescence.

The inner centromere protein encoded by the INCENP gene is phosphorylated by Cdk1 (cyclin dependent kinase 1) in mitosis. It is found to be associated with Plk1 (Polo-like kinase) in a polo-box–dependent manner. Plk1 is a serine/threonine kinase that functions in cell cycle, especially in mitosis. INCEP participates in the localization of Bub1 (Budding uninhibited by benzimidazole 1), a serine/threonine kinase involved in spindle checkpoint, to kinetochore. This, in turn, promotes the recruitment of Plk1 to kinetochores. Phosphorylated INCENP stimulates the activity of Aurora B kinase at centromeres and the central spindle that is important for chromosome segregation and cytokinesis, respectively.
Studies in knockout mice established INCENP (inner centromere protein) as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

rabbit

200

unconjugated

IgG fraction of antiserum

primary antibodies

polyclonal

buffered aqueous solution

antigen ~106 kDa

rat, human, mouse

indirect immunofluorescence: 1:100 using HeLa cells
microarray: suitable
western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 and rat PC12 cells
western blot (chemiluminescent): 1:5,000 using nuclear extract of human HeLa cells

Q9NQS7

dry ice

−20℃

human ... INCENP(3619)
mouse ... Incenp(16319)
rat ... Incenp(293733)

200 μL in glass bottle

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WGK 2

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)