Anti-l-Afadin antibody produced in rabbit

MFCD03455360

NA.41

Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .

Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.

Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.

Anti-l-Afadin antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence experiments
• immunocytochemistry
• immunohistochemistry

AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~200 kDa

mouse, human, rat, canine

antibody small pack of 25 μL

immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.
indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells.
microarray: suitable
western blot: 1:2,000 using extract of rat brain

dry ice

−20℃

human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)

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