Anti-NAA25 antibody produced in rabbit

HPA039322    Human Protein Atlas characterization data

The gene NAA25 (N(α)-acetyltransferase 25) is mapped to human chromosome 12. The encoded protein is an auxiliary subunit of the NatB (N-terminal acetyltransferase) complex.

N(alpha)-acetyltransferase 25, NatB auxiliary subunit recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

The NatB (N-terminal acetyltransferase) complex is responsible for the acetylation of N-terminal methionines in proteins during de novo protein synthesis. It is also responsible for the N-terminal acetylation of tropomyosin, thereby stabilizing and regulating actin remodeling. NAA25 (N(α)-acetyltransferase 25) is required for actin remodeling and cell motility. It regulates Rictor and controls the activity of mTORC2 (mammalian target of rapamycin complex 2). These events modulates Akt (protein kinase B), PKCα (protein kinase C α), and FoxO1 (forkhead box protein O1) activities, thereby influencing cellular homeostasis, cell motility and metabolic events. NAA25 suppresses the induction of autophagy via Akt phosphorylation, thereby stimulating polyQ (polyglutamine) aggregation. Polymorphism in the NAA25 gene region is associated with juvenile idiopathic arthritis.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST80992.

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

rabbit

100

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

Prestige Antibodies^® Powered by Atlas Antibodies

buffered aqueous glycerol solution

human

antibody small pack of 25 μL

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:50-1:200

TGKRFIEKDIQYPFLGPVPTRMGGFFNSGCSQCQISSFYLVNDIYELDTSGLEDTMEIQERIENSFKSLLDQLKDVFSKCKGDLL

Q14CX7

wet ice

−20℃

human ... NAA25(80018)

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10 - Combustible liquids

WGK 1