Anti-NBS1 (Nibrin) antibody produced in rabbit

MFCD07784851

NA.41

Nibrin (NBS1) is made up of 754 amino acids.
NBS1 (Nibrin), also known as p95 protein of the meiotic recombination 11 homolog 1/DNA repair protein RAD50 (MRE11/RAD50) complex. NBS1 contains two domains found in the cell cycle checkpoint proteins forkhead-associated domain (FHA) and an adjacent breast cancer carboxyterminal domain (BRCT). NBS1 is one of the protein components of the double-strand break repair complex NBS1/MRE11/p50.

synthetic peptide corresponding to amino acids 692-706 of mouse NBS1 (nibrin), conjugated to KLH via an N-terminal added cysteine residue.

Anti-NBS1 (Nibrin) antibody has been used:

• in immunofluorence
• in immunoblotting
• in immunoprecipitation

NBS1 (Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with Nijmegen breakage syndrome (NBS). The majority of NBS patients bear a five base pairs deletion that leads to truncated NBS1, called 657del5. p95/NBS1 (nibrin) deficiency abrogates the formation of the MRE11/RAD50 ionizing radiation-induced foci, revealing a molecular link between DSB repair and cell cycle checkpoints activated by DNA damage.
Nibrin (NBS1) is involved in stabilizing genomes and has a role in development of cancers. It also takes part in the repair mechanism after double strand breaks in the DNA.

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen 95 kDa (also recognizes 100 kDa band)

rat, chimpanzee, human, canine

immunoprecipitation (IP): 5-10 μg
indirect immunofluorescence: 5-10 μg/mL using Hela cells, fixed with paraformaldehyde/triton
microarray: suitable
western blot: 1-2 μg/mL using MCF7 nuclear extracts

O60934

dry ice

−20℃

human ... NBN(4683)
mouse ... Nbn(27354)
rat ... Nbn(85482)

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