Anti-PAK3 antibody produced in rabbit

MFCD01633961

NA.44

p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
Anti-PAK3 specifically reacts with mouse and rat PAK3 (65 kDa). It reacts weakly with human PAK3.

synthetic peptide corresponding to amino acids 1-13 of the N-terminal of mouse p21 activated kinase 3 (PAK3).

Anti-PAK3 may be used at a concentration of 4 μg to immunoprecipitate PAK3 from 0.5-1 mg mouse NIH3T3 total cell lysate. It may be used at a concentration of 1-2 μg/mL for detection in total mouse NIH3T3 lysate by immunoblotting.

Solution in 0.07 M Tris-glycine, pH 7.4, containing 30% glycerol and 0.035% sodium azide.

rabbit

200

unconjugated

IgG fraction of antiserum

primary antibodies

polyclonal

buffered aqueous glycerol solution

antigen 65 kDa

mouse, rat

immunoprecipitation (IP): 4 μg using 0.5 mg of mouse brain cytosol
western blot (chemiluminescent): 2 μg/mL using mouse 3T3 fibroblasts extract or mouse brain extract

Q62829

wet ice

−20℃

mouse ... Pak3(18481)
rat ... Pak3(29433)

12 - Non Combustible Liquids

WGK 3

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter