Anti-Palladin antibody produced in rabbit

NA.41

Palladin is a phosphoprotein, which is widely expressed in vertebrate cells and tissues. It is expressed as three major isoforms of 90, 140, and 200 kDa. Palladin consists of a proline-rich region in the NH₂-terminal half of the protein and three tandem Ig C2 domains in the COOH-terminal.
Palladin exists in humans as 7 different isoforms and is a component of actin-containing microfilaments.
Palladin is a microfilament-associated phosphoprotein that is known to colocalize with alpha-actinin at cell-cell junctions, stress fibres, embryonic Z-lines, and focal adhesions. Palladin abnormalities have been linked to familial pancreatic cancer.
Rabbit anti-Palladin antibody binds to human palladin. Detection of the palladin band by immunoblotting is specifically inhibited by the immunizing peptide.

Rabbit anti-palladin antibody can be used for immunoprecipitation (5-10μg), indirect immunofluorescence (2-5μg/mL) and western blot (0.5-1.0μg/mL) using HeLa cells.

Palladin controls cell shape, adhesion, and contraction. It interacts with the actin-associated proteins α-actinin, VASP, ezrin, and profilin, suggesting that it is required for normal actin cytoskeleton organization. The Ig3 domain of palladin binds to F-actin. Its expression is required for normal mammalian embryogenesis since its inactivation leads to embryonic lethality.

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~140/90 kDa

human

~1.0 mg/mL

immunoprecipitation (IP): 5-10 μg using lysates of HeLa cells
indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
western blot: 0.5-1.0 μg/mL using whole extracts of human HeLa cells

Q8WX93

dry ice

−20℃

human ... PALLD(23022)

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12 - Non Combustible Liquids