Anti-phospho-c-Kit (SCFR) (pTyr⁷³⁰) antibody produced in rabbit

MFCD06411871

NA.41

Mast cells play an important role in innate immunity or the response of the body to invading pathogens. The functions of mast cells are regulated by a number of surface receptors; c-Kit (Stem cell factor receptor, SCF R; CD117) being the most critical. The signalling triggered by c-Kit results in mast cell activation in response to antigens. The response involves tyrosine phosphorylation by specific tyrosine kinases that drive the downstream signalling process. The secondary pathways activated by c-Kit include Akt, mTOR, MAPK, Sos-Grb2 and c-Jun. Phosphorylation of tyrosine 730 is required for the binding of PLCγ leading to activation of various signalling pathways In addition to development of mast cells, c-Kit also regulates their subsequent maturation and homing to the residence sites. Constitutive activation by SCF R is implicated in myeloproliferative discorders, systemic mastocytosis and gastrointestinal stomal cell tumors
The antibody detects human c-Kit pTyr⁷³⁰. Mouse, rat and cow (100% homologous), dog (92% homologous), chicken (85%) are expected to react.

synthetic phosphopeptide derived from the region of human c-Kit that contains tyrosine 730.

Anti-phospho-c-Kit (SCFR) pTyr⁷³⁰ antibody may be used for immunoblotting at a working concentration of 0.1-1.0 μg/ml using M07e cells.

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

rabbit

100

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

rat, human, mouse

western blot: 0.1-1.0 μg/mL using M07e cells

P10721

dry ice

−20℃

human ... KIT(3815)
mouse ... Kit(16590)
rat ... Kit(64030)

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