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Anti-phospho-c-Raf (pSer338/pTyr340) antibody produced in rabbit
| 产品编号： | 3384255 |
| 规格： | affinity isolated antibody, buffered aqueous glycerol solution |
| 包装规格： | 1 VIAL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD06411878 |
| NACRES | NA.44 |
| General description【一般描述】 | c-Raf or Raf-1 belongs to the Raf family of kinases that have regulatory functions in cell growth, differentiation, growth factor receptor stimulation via the MAPK signalling. The other members of the family are A-Raf and B-Raf. All the isoforms have unique as well as overlapping regulatory functions. c-Raf kinase is the first identified downstream effector kinase of Ras, and is the activator of MEK which in turn activates ERK. Phosphorylation of c-Raf at Ser338 and Tyr340 regulates its biological function. Anti-phospho-c-Raf [pSer338/pTyr340] detects human c-Raf [pSer338/pTyr340]hosphorylated at [pSer338/pTyr340]er338 and [pSer338/pTyr340]yr340 (74 kDa). Mouse and [pSer338/pTyr340]at c-Raf (100% homology) are [pSer338/pTyr340]xpected [pSer338/pTyr340]o cross react. |
| Immunogen【免疫原】 | synthetic phosphopeptide derived from the region of human c-Raf that contains serine 338 and tyrosine 340. |
| Application【应用】 | The antibody may be used to detect phospho-c-Raf (Ser338/Tyr340) by immunoblotting at working dilution of 1:1000 in cell lysates from HEK293 cells transfected with c-Raf. Detection was also possible in spermatozoa of mice at 1:1000 dilution. |
| Physical form【外形】 | Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous glycerol solution |
| species reactivity | mouse, rat, human |
| technique(s) | western blot: 1:1,000 using cell lysates from Hek293 cells transfected with c-Raf, or co-transfected with c-Raf, Ras[V12] and Rac1. |
| UniProt accession no.【UniProt登记号】 | P04049 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... RAF1(5894) mouse ... Zhx2(387609) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
