Anti-phospho-DARPP32 (pThr⁷⁵) antibody produced in rabbit

MFCD08705496

NA.41

DARPP32 is a phosphoprotein with a molecular mass of 32kDa. It is regulated by dopamine (DA) and cAMP and is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. DARPP32 is a target for the actions of dopamine.

synthetic phosphopeptide corresponding to amino acids residues surrounding the DARPP32 phosphorylated on threonine 75.

Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit is suitable for dot blot and western blot analysis at a working dilution of 1:1000 using rat caudate lysates.

Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32. Phosphorylation of DARPP32 plays an important role in the regulation of dopaminergic neurotransmission. The activity of this protein has an important role in the actions of alcohol, caffeine and Prozac^®.

Solution at 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

The labeling by the antibody to DARPP32 Thr⁷⁵ is markedly increased by okadaic acid treatment.

Prozac is a registered trademark of Eli Lilly and Co.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous glycerol solution

sufficient for 10 blots

antigen 32 kDa

mouse, human

dot blot: 1:1,000
western blot: 1:1,000

Q9UD71

wet ice

−20℃

human ... PPP1R1B(84152)
mouse ... Ppp1r1b(19049)

DARPP32 phosphorylated on Thr⁷⁵ acts as an inhibitor of PKA.

13 - Non Combustible Solids

WGK 2

Eyeshields, Gloves, type N95 (US)