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Anti-phospho-Epidermal Growth Factor Receptor (pTyr992) antibody produced in rabbit
| 产品编号： | 3384584 |
| 规格： | affinity isolated antibody, buffered aqueous glycerol solution |
| 包装规格： | 1 VIAL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho-Epidermal Growth Factor Receptor (pTyr992) antibody produced in rabbit
Anti-phospho-EGFR (pTyr992)
基本信息
| MDL number | MFCD05665244 |
| NACRES | NA.41 |
| General description【一般描述】 | The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis. Tyrosine 992 of EGF R is autophosphorylation site and required for increasing the signalling capacity of EGF R Anti-phospho-EGFR [pTyr992] specifically recognizes human EGFR phosphorylated at tyrosine 992 (~185 kDa). Cross reactivity may be observed with mouse, rat and chicken. |
| Immunogen【免疫原】 | synthetic phosphopeptide derived from the region of human EGFR that contains tyrosine 992. The sequence is conserved in mouse and rat with 100% homology. Chicken EGFR is 80% homologous. |
| Application【应用】 | Anti-phospho-EGFR [pTyr992] antibody may be used at a working dilution of 1:1000 for immunoblotting using A431 cells. |
| Physical form【外形】 | Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+) containing 50% glycerol with 1 mg/mL BSA (protease and IgG-free) and 0.05% sodium azide. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous glycerol solution |
| mol wt【分子量】 | antigen ~185 kDa |
| species reactivity | human, rat (predicted), mouse (predicted), chicken (predicted) |
| technique(s) | western blot: 1:1,000 using human epidermoid carcinoma A431 cells |
| UniProt accession no.【UniProt登记号】 | P00533 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... EGFR(1956) mouse ... Egfr(13649) rat ... Egfr(24329) |
