Anti-phospho-YAP1 (pTyr³⁵⁷) 兔抗

MFCD04118707

NA.44

Yes-associated protein 1 (YAP1) is a 65 kDa and a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with Src-family tyrosine kinase c-Yes. In addition to a SH3-binding motif, YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 binds to p73 through its WW domain and the PPPY motif of p73. This interaction is required for the ability of YAP1 to co-activate p73-responsive genes.

Anti-phospho-YAP1 pTyr³⁵⁷ antibody is specific for human phospho-YAP1 pTyr³⁵⁷. The product may react with chicken and mouse phospho-YAP1 pTyr³⁵⁷. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.

synthetic peptide corresponding to amino acids 351-362 (pTyr³⁵⁷) located in the C-terminal region of human YAP1. The sequence is identical in chicken YAP1 and highly conserved in mouse YAP1 (single amino acid substitution).

Anti-phospho-YAP1 (pTyr357) antibody produced in rabbit has been used in western blotting and immunofluorescence.

YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development
Anti-phospho-YAP1 pTyr³⁵⁷ antibody is specific for human phospho-YAP1 pTyr³⁵⁷. The product may react with chicken and mouse phospho-YAP1 pTyr³⁵⁷. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.
Inhibition of Akt potentiates the nuclear re-localization of YAP1 to induce apoptosis by p73.

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~65 kDa

human

antibody small pack of 25 μL

~1.5 mg/mL

indirect immunofluorescence: 5-10 μg/mL using HEK-293 cells co-transfected with human YAP1 and c-Abl
western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and human c-Abl

P46937

dry ice

−20℃

chicken ... YAP1(396171)
human ... YAP1(10413)
mouse ... Yap1(22601)

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10 - Combustible liquids

WGK 1