Anti-Phosphotyrosine (pY-256) antibody produced in rabbit

MFCD06411901

NA.44

Protein phosphorylation is a post-translational modification that regulates signal transduction. Phosphorylation of tyrosine residues in proteins has been associated with oncogenesis, cell growth and survival . Phosphotyrosines have also been linked to other cellular functions. For instance, tyrosine 256 phosphorylation in atypical protein kinase C enables its entry into the nucleus of cells . Anti-Phosphotyrosine pTyr²⁵⁶ binds to tyrosine phosphorylated proteins. In immunoblotting, the antibody detects [pTyr256] in random peptides and phospho-L-tyrosine, but does not react with phospho-L-serine, phospho-L-threonine and L-tyrosine.

phospho-L-tyrosine containing random peptide pY-256, conjugated to KLH.

Anti-Phosphotyrosine (pY-256) antibody is suitable for use in western blot (1:1000) . It may also be used in indirect immunofluorescence (5-10 μg/mL using A431 stiμLated by human EGF), immunoblotting and immunocytochemistry.

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

indirect immunofluorescence: 5-10 μg/mL using A431 stimulated by human EGF.
western blot: 0.5-1.0 μg/mL using cell extract of A431 stimulated by human EGF.

−20℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.