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产品别名
Anti-POLR3A antibody produced in rabbit
RPC155
HLD7
hRPC155
RPC1
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | liquid |
mol wt【分子量】 | 156 kDa |
species reactivity | human |
concentration【浓度】 | 0.89 mg/mL |
technique(s) | immunocytochemistry: suitable immunofluorescence: suitable immunoprecipitation (IP): suitable western blot: 500-3000 |
UniProt accession no.【UniProt登记号】 | O14802 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... POLR3A(11128) |
基本信息
Immunogen【免疫原】 | Recombinant fragment corresponding to a region within amino acids 182 and 482 of POLR3A (Uniprot ID#O14802) |
Application【应用】 | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
Biochem/physiol Actions【生化/生理作用】 | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. |
Features and Benefits【特点和优势】 | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
Physical form【外形】 | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Pictograms【象形图】 | GHS07 |
Signal word【警示用语:】 | Warning |
Hazard Statements | H317 - H412 |
Precautionary Statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
Hazard Classifications【危险分类】 | Aquatic Chronic 3 - Skin Sens. 1 |
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |