Anti-RAB25 (C-Terminal) antibody produced in rabbit

NA.41

RAB25 (Ras-related protein 25) belongs to the Rab family. It is located on chromosome 1q22. It is also called as Rab11c (Ras-related protein Rab11C). It is present in polarized epithelial cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Anti-RAB25 (C-Terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Rab (Ras related in brain) GTPases are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Through their effector proteins, Rab GTPases helps to modulate vesicle formation, actin- and tubulin-dependent vesicle movement and membrane fusion. Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Rab25 is associated with the apical recycling system of epithelial cells. Rab25 is associated with the progression and aggressiveness of ovarian and breast cancers.
In glioblastoma multiforme cells, downregulation of RAB25 (Ras-related protein 25) blocks the in vitro cytotoxicity and in vivo antitumor actions. Hence, it is known to play a major role in tumorigenesis. Upregulation of Rab25 induces the invasion and multiplication of hepatocellular carcinoma cell.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~25 kDa

human

~1.0 mg/mL

immunoprecipitation (IP): 5-10 μg using lysates of A431 cells
indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
western blot: 0.2-0.5 μg/mL using lysates of HEK-293T cells over expressing human Rab25

P57735

dry ice

−20℃

human ... RAB25(57111)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1