抗 兔 IgG(全分子)-碱性磷酸酶 山羊抗

MFCD00162782

NA.46

Binds all rabbit Igs.
Immunoglobulin G (IgG) belongs to the immunoglobulin family. It is widely expressed and consists of a gamma (γ) heavy chain in the constant (C) region. IgG is a monomer with a molecular weight of about 150 kDa. The structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively. The primary structure of the antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The Fc fragment contains the C_H2 and C_H3 domains of the antibody molecule.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.

purified rabbit IgG

Lysates of human fibroblasts and Hela cells were analyzed by western blot using alkaline phosphatase-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:30000 dilution.
Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for immunohistochemistry and western blot applications. The antibody can also be used for direct ELISA at 1:45,000.
通过 ELISA 法分析 PADI4 对抗凝血酶的瓜氨酸化作用，使用碱性磷酸酶标记的羊抗兔 IgG 作为在0.05M碳酸盐/碳酸氢盐缓冲液(Ph 9.6)中以1：5000稀释的二次稀释剂。

Antibody adsorbed with human IgG.

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide as preservative

200

goat

alkaline phosphatase conjugate

affinity isolated antibody

secondary antibodies

polyclonal

buffered aqueous solution

rabbit

direct ELISA: 1:45,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:100,000-1:200,000 using detecting actin in total cell extract of HeLa cells (5-10 μg per well)

wet ice

2-8℃

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10 - Combustible liquids

WGK 3