Anti-Rabbit IgG (whole molecule)-FITC antibody produced in sheep

MFCD00162786

NA.46

Sheep polyclonal anti-Rabbit IgG (whole molecule)−FITC antibody binds normal rabbit serum and rabbit IgG. It does not cross react with human IgG.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Sheep polyclonal anti-Rabbit IgG (whole molecule)−FITC antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids by fluorescent techniques.
Anti-Rabbit IgG (whole molecule)-FITC antibody produced in sheep has been used in:

• immunocytochemistry
• immunofluorescence
• immunohistochemistry

Antibody adsorbed with human IgG.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

200

sheep

FITC conjugate

affinity isolated antibody

secondary antibodies

polyclonal

buffered aqueous solution

protect from light

direct immunofluorescence: 1:160
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

−20℃

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