抗-SPAG6 兔抗

HPA038440    Human Protein Atlas characterization data

Sperm associated antigen 6 (SPAG6) is a novel cancer-testis antigen, encoded by the gene mapped to human chromosome 10p11.2–p12. The encoded protein is a mammalian orthologue of Chlamydomonas PF16. SPAG6 is localized to the axoneme central apparatus.

sperm associated antigen 6 recombinant protein epitope signature tag (PrEST)

Anti-SPAG6 antibody produced in rabbit has been used in immunohistochemistry (IHC).
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Sperm associated antigen 6 (SPAG6) is an essential protein involved in the assembly and structural integrity of the sperm tail axoneme. It also plays a crucial role in flagellar motility. Knockdown of SPAG6 gene inhibited the growth of malignant myeloid cell lines SKM-1 and K562 via activation of TNF-related apoptosis-inducing ligand (TRAIL) signaling pathway. Elevated expression of SPAG6 is observed in CD34⁺ cells of myelodysplastic syndromes (MDSs) and acute myelogenous leukemia (AML) patients.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST80170.

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

rabbit

100

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

Prestige Antibodies^® Powered by Atlas Antibodies

buffered aqueous glycerol solution

human

antibody small pack of 25 μL

orthogonal RNAseq
recombinant expression
Learn more about Antibody Enhanced Validation

immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:500-1:1000

AVPLLVLCIQEPEIALKRIAASALSDIAKHSPELAQTVVDAGAVAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEA

O75602

wet ice

−20℃

human ... SPAG6(9576)

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