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Anti-TSG101 (C-terminal) antibody produced in rabbit
产品编号: | 3403051 |
规格: | ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-TSG101 (C-terminal) antibody produced in rabbit
Anti-tumor susceptibility gene 101
基本信息
MDL number | MFCD08705529 |
NACRES | NA.41 |
General description【一般描述】 | Tumor susceptibility gene 101 encodes a multi-domain binding protein TSG101 which is 46 kDa. It is expressed in all tissues throughout development and is essential for both embryonic development and normal cell growth. It has an N-terminal UEV (N-terminal ubiquitin E2 variant) domain. Subcellular localization of TSG101 fluctuates in a cell-cycle dependent manner being localized in the nucleus and Golgi complex during interphase and in mitotic spindles and centrosome during mitosis. |
Immunogen【免疫原】 | synthetic peptide corresponding to amino acids 371-390 located at the C-terminus of human TSG101, conjugated to KLH. This sequence is identical in rat and mouse TSG101. |
Application【应用】 | Anti-TSG101 (C-terminal) antibody has been used in immnunoblotting and western blotting. |
Biochem/physiol Actions【生化/生理作用】 | TSG101 mediates a variety of cellular functions including transcriptional regulation, ubiquitination, endosomal trafficking, proliferation, and cell survival. It has a central role in protein sorting during endocytosis. Human TSG101 is also required for the budding of several enveloped RNA viruses including human immunodeficiency (HIV) and Ebola viruses. TSG101 is involved in the regulation of the p53/MDM2 (proto-oncogene) pathway by affecting E3 ubiquitin-protein ligase MDM2 (MDM2) stability. The N-terminal UEV domain binds to a P(T/S)AP sequence of HIV-1 p6 Gag and Ebola Vp40 proteins required for budding. The proline-rich domain was found to act as an activation domain in transcriptional regulation. The C-terminal coiled-coil (CC2) domain was predicted to interact with the cell growth regulating protein stathmin and is involved in potential co-repressor activity. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen 46 kDa (additional band of ~105 kDa may be observed in some preparations) |
species reactivity | human, mouse, rat |
concentration【浓度】 | ~1.5 mg/mL |
technique(s) | western blot: 0.4-0.8 μg/mL using rat brain extract (S1 cytosolic fraction) western blot: 1-2 μg/mL using cell lysates of mouse fibroblast NIH3T3 cells and human A431 cells |
UniProt accession no.【UniProt登记号】 | Q99816 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... TSG101(7251) mouse ... Tsg101(22088) rat ... Tsg101(292925) |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |