Anti-WAS antibody produced in rabbit

HPA002022    Human Protein Atlas characterization data

Wiskott-Aldrich Syndrome protein (WAS) is a hematopoietic-specific protein. It is expressed in lymphocytes, spleen, and thymus. WAS mainly is involved in the stimulation of the actin nucleation factor, Arp2/3, complex for actin polymerization and actin cytoskeleton architecture stabilization in hematopoietic cells. It possess a C-terminal tripartite VCA (verprolin,central, acidic) domain.

Wiskott-Aldrich syndrome protein recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

In WAS (Wiskott-Aldrich Syndrome), protein the VCA (verprolin,central, acidic) domain binds to the Arp (actin-related protein)-2/3 complex via the V region to activate the complex as well as to stimulate actin polymerization. WAS has also a CRIB (Cdc42- and Rac-interactive binding) domain for binding to active GTP-bound form of Cdc42 (cell division cycle 42). The WAS activity is regulated by formation an auto-inhibited loop structure. It also plays a vital role in cytoplasmic tyrosine kinase pathway in B-lineage cells.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST78237.

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC

rabbit

100

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

Prestige Antibodies^® Powered by Atlas Antibodies

buffered aqueous glycerol solution

human

antibody small pack of 25 μL

orthogonal RNAseq
Learn more about Antibody Enhanced Validation

immunohistochemistry: 1:200- 1:500

LQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKK

P42768

wet ice

−20℃

human ... WAS(7454)

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