Anti-WASH1 antibody produced in rabbit

NA.41

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog) is a new member of the WASP family. Similar to other WASP family members, it contains a C-terminal for ′WH2, connecting and acidic′ (WCA) domain that binds to Actin related protein 2/actin related protein 3 (Arp2/3). In addition, WASH1 also contains a short proline-rich region, a unique N-terminal domain termed WASH-homology domain (WAHD1), and a tubulin-binding region.

synthetic peptide corresponding to an internal region of human WASH1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Anti-WASH1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), is a nucleation-promoting factor at the surface of endosomes. It recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of endosomes. WASH1 forms part of a multiprotein complex composed of FAM21, KIAA1033 strumpellin and WASH-interacting protein (SWIP) and coiled coil domain containing 53 (CCDC53). It associates with tubulin and localizes to early and recycling endosomes, where together with the Arp2/3 complex and actin, it is required for maintaining the shape of the endosomal compartment and the regulation of the retrograde transport.

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

rabbit

200

unconjugated

affinity isolated antibody

primary antibodies

polyclonal

buffered aqueous solution

antigen ~72 kDa

human, mouse, rat

~1.0 mg/mL

immunoprecipitation (IP): 5-10 μg using lysates of rat NRK cells.
western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing mouse WASH1.

dry ice

−20℃

human ... WASH1(100287171)
mouse ... ORF19(68767)
rat ... 367328(Wash2)

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12 - Non Combustible Liquids