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三磷酸腺苷双磷酸酶 来源于马铃薯

Apyrase from potatoes
产品编号:3409011
规格:Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein
CAS NO:9000-95-7
包装规格: 100 UNITS, 200 UNITS, 500 UNITS, 1 KU, 2 KU
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
3409011 100 UNITS1840
3409011 200 UNITS3180
3409011 500 UNITS5400
3409011 1 KU8810
3409011 2 KU15890
产品别名

9000-95-7

三磷酸腺苷双磷酸酶 来源于马铃薯

Apyrase from potatoes

Adenosine 5′-triphosphatase

Adenosine 5′-diphosphatase

腺苷 5′-二磷酸酶

腺苷 5′-三磷酸酶

基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】
3.6.1.5 ( BRENDA | IUBMB )
EC number【EC 号】
232-569-8
MDL number
MFCD00130542
NACRES
NA.32
Application【应用】
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.
在多种马铃薯普通栽培种中至少发现了两种同工酶:一种具有高 ATP 酶/ADP 酶比率(约 10),另一种该比率较低(约 1)。
反应:ATP → ADP+Pi → AMP+2Pi
Biochem/physiol Actions【生化/生理作用】
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Unit Definition【单位定义】
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 ℃.
Physical form【外形】
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Preparation Note【制备说明】
按 ATP 酶单位销售。
Other Notes【其他说明】
该等级具有低 ATP 酶/ADP 酶比率。
产品性质
Quality Level【质量水平】
400
type【类型】
Grade VII
form【形式】
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≥50 % of base activity ADPase
composition【组成】
Protein, 25-60%
solubility【溶解性】
H2O: soluble 1.0 mg/mL
application(s)
diagnostic assay manufacturing
foreign activity【异质活性】
Acid Phosphatase ≤2% of base activity
storage temp.【储存温度】
−20℃
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
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