β-葡萄糖醛酸酶 来源于大肠杆菌

3.2.1.31 ( BRENDA | IUBMB )

MFCD00130629

NA.54

β-Glucuronidase from Escherichia coli is similar to human glucuronidase enzyme and corresponds to molecular weight close to 69-71 kDa and has an pH optimum of 6.5-7.5. It belongs to family-2 glycosyl hydrolase and has active site residues glutamic acid 394, tyrosine 468 and glutamic acid 504.

β-Glucuronidase from E. coli is used for the enzymatic hydrolysis of b-glucuronides in urine and other fluids. It has a high rate of hydrolytic activity and may be useful for determining the presence of androsterone, 17-hydroxycorticosteroids, and estriol in urine.
The optimal conditions for the enzymatic hydrolysis of α-hydroxytriazolam, one of the major metabolites of triazolam in human urine, were determined using β-glucuronidase Type IX-A.
It is used as a reporter gene in GUS assays to monitor gene expression.
β-Glucuronidase from Escherichia coli has been used in the enzymatic cleavage and activation of glucuronide prodrugs in non-small cell lung cancer cells, U87 human glioblastoma cell line, in A549 (human lung adenocarcinoma) and KB (human oral squamous carcinoma).
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据，以优化不同来源的酶对不同药物类别的水解条件，并使用色谱纯化酶降低酯酶活性的影响，从而将6-MAM转化为吗啡
有效水解类固醇葡糖苷酸。

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
β-Glucuronidase catalyzes the hydrolysis of β-glucuronic acid residues from the non-reducing termini of glycosaminoglycans (GAGs). β-Glucuronidase is a potential candidate enzyme for gene-mediated enzyme prodrug therapy for glucuronide prodrugs. Use of β-Glucuronidase-albumin complex based drug delivery could be an effective therapeutic methodology for treating solid tumors.

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 ℃ at the pH 6.8 (30 min assay).

Highly purified solution in 50% glycerol

200

aqueous glycerol solution

≥5,000,000 units/g protein, pH 6.8 (biuret)

69-71 kDa

wet ice

−20℃

25000 units in glass bottle

GHS08

Danger

H317 - H334

P261 - P280 - P342 + P311

Resp. Sens. 1 - Skin Sens. 1

12 - Non Combustible Liquids

WGK 3