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产品性质
| biological source【生物来源】 | fish trunk |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | 2n = 48 |
| morphology【形态学】 | Fibroblast |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Fish Bluegill fry caudal trunk |
| Cell Line Description【细胞系描述】 | Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15℃ to 33℃. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs. |
| Application【应用】 | Virus studies, targets for bacterial cytotoxins |
| Culture Medium【培养基】 | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (pH7.4 - 7.5). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin/EDTA; 5% CO2; 23℃. A replication capacity of up to 50 passages is expected. Fish cell lines detach easily during transit if the culture is too young. After resuscitation, split cells |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
