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BRIN-BG7
产品别名
BRIN-BG7
BRIN
产品性质
| biological source【生物来源】 | rat pancreas (islets) |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Modal chromosome number 66 up to passage 50 |
| morphology【形态学】 | Epitheloid |
| products【产品】 | BRIN-BG7 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG7 cells express the GLUT2 glucose transporter. |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
基本信息
| Cell Line Origin【细胞系来源】 | A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line |
| DNA Profile【DNA图谱分析】 | Not specified |
| Culture Medium【培养基】 | RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
产品说明
| Disclaimer【免责声明】 | This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. |
