CAR Knockout HepaRG^TM Cells

NA.81

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG CAR KO cells express the major xenobiotic sensors (PXR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

See technical bulletin for detailed protocols

Zinc finger nucleases (ZFN) mediated Knock-out of NR1I3 (CAR) gene.

• The frame-shift mutation of NR1I3 gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

• Sigma′s HepaRG CAR Knockout (KO) allows conclusive identification of CYP2B6 induction through CAR activation.

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company

200

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

liquid

−196℃

human ... ABCB1(1346307)

12 - Non Combustible Liquids

WGK 3