Carboxypeptidase A-Agarose

3.4.17.1 ( BRENDA | IUBMB )

MFCD00130718

NA.54

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.
Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 ℃.

Suspension in 2.0 M (NH₄)₂SO₄, pH 7

200

enzyme from bovine pancreas

ammonium sulfate suspension

≥6 units/mL packed gel, 25 ℃

~35,250

beaded agarose

2-8℃

GHS07,GHS08

Danger

H315 - H319 - H334 - H335

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Respiratory system

10 - Combustible liquids

WGK 1