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产品性质
| biological source【生物来源】 | human blood |
| growth mode【生长模式】 | Suspension |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Lymphoblast |
| products【产品】 | Surface Immunoglobulin ( Ig), MHC Class II |
| receptors【受体】 | Fc, complement |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human lymphoblast |
| Cell Line Description【细胞系描述】 | Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2. |
| Application【应用】 | T cell replacement factor (TRF) assays, B cell differentiation studies |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X,Y CSF1PO: 10,11 D13S317: 12 D16S539: 12 D5S818: 11,12 D7S820: 10,12 THO1: 7,9.3 TPOX: 8,9 vWA: 16,17 |
| Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
