您的位置：首页 > 产品中心 > Chaperonin 60 from Escherichia coli
Chaperonin 60 from Escherichia coli
| 产品编号： | 3424645 |
| 规格： | >95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder |
| 包装规格： | 1 MG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Chaperonin 60 from Escherichia coli
GROEL
基本信息
| MDL number | MFCD00163256 |
| NACRES | NA.32 |
| Application【应用】 | Chaperonin 60 from Escherichia coli has been used:
|
| Biochem/physiol Actions【生化/生理作用】 | Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese. The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained. |
| Physical form【外形】 | Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer. |
产品性质
| biological source【生物来源】 | Escherichia coli |
| Quality Level【质量水平】 | 200 |
| recombinant【重组】 | expressed in E. coli overproducing strain |
| Assay【测定】 | >95% (SDS-PAGE) |
| form【形式】 | lyophilized powder |
| technique(s) | electron microscopy: suitable mass spectrometry (MS): suitable |
| UniProt accession no.【UniProt登记号】 | P10809 |
| storage temp.【储存温度】 | 2-8℃ |
| Gene Information | human ... HSPD1(3329) |
| packaging【包装】 | 1 mg in serum bottle Package size based on protein content. |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
